| 出品公司: | BD Biosciences |
|---|---|
| 载体名称: | pAcHLT C |
| 质粒类型: | 昆虫细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 8108 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pAcHLTC载体基本信息
pAcHLTC载体图谱质粒图谱和多克隆位点信息
pAcHLTC载体简介
pAcHLTC载体序列
LOCUS pAcHLT C 8108 bp ds-DNA circular SYN 19-APR-2014
DEFINITION Baculovirus transfer vector for expressing proteins with an
N-terminal 6xHis-PKA-thrombin cassette. For other reading frames,
use pAcHLT A or pAcHLT B.
ACCESSION .
VERSION .
KEYWORDS pAcHLT C
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8108)
AUTHORS BD Biosciences
TITLE Direct Submission
JOURNAL Exported Wednesday, February 5, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8108
/organism="synthetic DNA construct"
/lab_host="Insect Cells"
/mol_type="other DNA"
misc_recomb 1269..2096
/note="baculovirus recombination region (lef2/ORF603)"
/note="contains ORF603 and part of lef2"
CDS 1269..1393
/codon_start=3
/product="baculovirus late expression factor 2"
/note="lef2"
/translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
CDS complement(1431..2036)
/codon_start=1
/product="baculovirus ORF603 protein"
/note="ORF603"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
promoter 2100..2191
/gene="polh from Autographa californica"
/note="polyhedrin promoter"
/note="promoter for the baculovirus polyhedrin gene"
CDS 2230..2247
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 2254..2271
/codon_start=1
/product="recognition site for phosphorylation by protein
kinase A"
/note="PKA site"
/translation="RRASVA"
CDS 2281..2298
/codon_start=1
/product="thrombin recognition and cleavage site"
/note="thrombin site"
/translation="LVPRGS"
misc_feature 2313..2380
/note="MCS"
/note="multiple cloning site"
misc_recomb 2400..3858
/note="baculovirus recombination region (ORF1629)"
/note="contains part of ORF1629"
CDS complement(2517..3858)
/codon_start=2
/product="baculovirus capsid-associated protein"
/note="ORF1629"
/note="required for viral replication"
/translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
rep_origin 5231..5686
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5968..6072
/gene="bla"
/note="AmpR promoter"
CDS 6073..6933
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7104..7692
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatagat
2221 ccgatgggac atcatcatca tcatcacgga aggagaaggg ccagtgttgc ggcgggaatt
2281 ttggtccctc gtggaagccc aggactcgat ggcatatgct cgaggaattc aggcctccat
2341 gggagctcgc ggccgcctgc agggtacccc cgggagatct gtaccgactc tgctgaagag
2401 gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc accagacgca
2461 cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta catttattaa
2521 gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt taataattca
2581 ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct
2641 ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt tcgattagtt
2701 tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt ttcgctcaac
2761 gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt
2821 ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt atttctttca
2881 tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaataaagc ttggacatat
2941 ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca accctcgtcg
3001 ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt aattgtgtcg
3061 gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc tgattgcggg
3121 cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa cacgttagaa
3181 agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg cggcggtggt
3241 ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg cggaggcgga
3301 ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc tttaggcaac
3361 acagtcggca cctcaactat tgtactggtt tcgggcgccg tttttggttt gaccggtctg
3421 agacgagtgc gatttttttc gtttctaata gcttccaaca attgttgtct gtcgtctaaa
3481 ggtgcagcgg gttgaggttc cgtcggcatt ggtggagcgg gcggcaattc agacatcgat
3541 ggtggtggtg gtggtggagg cgctggaatg ttaggcacgg gagaaggtgg tggcggcggt
3601 gccgccggta taatttgttc tggtttagtt tgttcgcgca cgattgtggg caccggcgca
3661 ggcgccgctg gctgcacaac ggaaggtcgt ctgcttcgag gcagcgcttg gggtggtggc
3721 aattcaatat tataattgga atacaaatcg taaaaatctg ctataagcat tgtaatttcg
3781 ctatcgttta ccgtgccgat atttaacaac cgctcaatgt aagcaattgt attgtaaaga
3841 gattgtctca agctcggatc gatcccgcac gccgataaca agccttttca tttttactac
3901 agcattgtag tggcgagaca cttcgctgtc gtcgacgtac atgtatgctt tgttgtcaaa
3961 aacgtcgttg gcaagcttta aaatatttaa aagaacatct ctgttcagca ccactgtgtt
4021 gtcgtaaatg ttgtttttga taatttgcgc ttccgcagta tcgacacgtt caaaaaattg
4081 atgcgcatca attttgttgt tcctattatt gaataaataa gattgtacag attcatatct
4141 acgattcgtc atggccacca caaatgctac gctgcaaacg ctggtacaat tttacgaaaa
4201 ctgcaaaaac gtcaaaactc ggtataaaat aatcaacggg cgctttggca aaatatctat
4261 tttatcgcac aagcccacta gcaaattgta tttgcagaaa acaatttcgg cgcacaattt
4321 taacgctgac gaaataaaag ttcaccagtt aatgagcgac cacccaaatt ttataaaaat
4381 ctattttaat cacggttcca tcaacaacca agtgatcgtg atggactaca ttgactgtcc
4441 cgatttattt gaaacactac aaattaaagg cgagctttcg taccaacttg ttagcaatat
4501 tattagacag ctgtgtgaag cgctcaacga tttgcacaag cacaatttca tacacaacga
4561 cataaaactc gaaaatgtct tatatttcga agcacttgat cgcgtgtatg tttgcgatta
4621 cggattgtgc aaacacgaaa actcacttag cgtgcacgac ggcacgttgg agtattttag
4681 tccggaaaaa attcgacaca caactatgca cgtttcgttt gactggtacg cggcgtgtta
4741 acatacaagt tgctaaccgg cggccgacac ccatttgaaa aaagcgaaga cgaaatgttg
4801 gacttgaata gcatgaagcg tcgtcagcaa tacaatgaca ttggcgtttt aaaacacgtt
4861 cgtaacgtta acgctcgtga ctttgtgtac tgcctaacaa gatacaacat agattgtaga
4921 ctcacaaatt acaaacaaat tataaaacat gagtttttgt cgtaaaaatg ccacttgttt
4981 tacgagtaga attagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
5041 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
5101 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
5161 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
5221 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
5281 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
5341 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
5401 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
5461 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
5521 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
5581 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
5641 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
5701 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
5761 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
5821 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
5881 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
5941 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
6001 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
6061 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
6121 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
6181 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
6241 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
6301 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
6361 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
6421 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
6481 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
6541 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
6601 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
6661 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
6721 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
6781 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
6841 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
6901 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
6961 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
7021 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
7081 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
7141 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
7201 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
7261 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
7321 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
7381 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
7441 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
7501 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
7561 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
7621 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
7681 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
7741 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
7801 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
7861 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
7921 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
7981 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
8041 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
8101 tacgaatt
//
